ZFIN ID: ZDB-FIG-130320-27
Choi et al., 2013 - In vivo monitoring of cardiomyocyte proliferation to identify chemical modifiers of heart regeneration. Development (Cambridge, England)   140(3):660-666 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Day 4

Fig. 1 FUCCI zebrafish for visualizing cardiomyocyte proliferation. (A) Representative maximum intensity projection of the heart of a 4 dpf cmlc2:FUCCI transgenic larva, visualizing non-proliferating (cmlc2:mCherry-zCdt1, red) and proliferating (cmlc2:Venus-hGeminin, green) cardiomyocytes, as depicted in the schematic at the top. Cardiomyocytes with only the Venus-hGeminin signal (arrowheads) were considered to be proliferating. DAPI, blue. Scale bars: 50 μm. (B) Time course of total (red+ and green+) and proliferating (green+) cardiomyocytes from 2 to 6 dpf. Percentages of proliferating cardiomyocytes are indicated above bars. Data are represented as meanĀ±s.e.m. n=13-24 embryos per stage.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry pd57Tg/+; pd58Tg/+ standard conditions Day 4 cardiac muscle cell IFL
Venus pd57Tg/+; pd58Tg/+ standard conditions Day 4 cardiac muscle cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development