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ZFIN ID: ZDB-FIG-130220-2
Bensimon-Brito et al., 2012 - Distinct patterns of notochord mineralization in zebrafish coincide with the localization of Osteocalcin isoform 1 during early vertebral centra formation. BMC Developmental Biology   12(1):28 Full text @ BMC Dev. Biol.
Anatomical Terms:
Stage: Days 7-13

Fig. 3 Early mineralization stages of vertebral centra and association with cartilaginous arches. Lateral view of (a, a′) Collagen type II immunostaining in the vertebral column of a 5.9 mm TL zebrafish shows (a) protein accumulation mostly in the cartilaginous structures of the Weberian apparatus and of the caudal fin skeleton (arrows) but also in the notochord sheath (white arrowheads). (a′) Although less evident than in cartilage, the staining can be clearly identified in the notochord matrix in a segmented manner (white arrowheads). (b-c) Early mineralization can be detected through alizarin red S (ARed) staining viewed under fluorescent light. (b) Early stage “ring” centrum (arrowheads) and (b′) close up showing the mineralization fronts (white arrowheads) in 5.0 mm TL fish. (c) Caudal fin centrum mineralization with a basiventral origin (arrowhead) and (c′) close up presenting a uniform mineralization surface with no distinct mineralization fronts, here represented by a 6.5 mm TL fish. Scale bars (a): 1 mm; (a′) 0.1 mm; (b,c): 0.15 mm; (b′, c′) 30 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-col2a IHC WT standard conditions Days 7-13 caudal fin skeleton
IHC Days 7-13 notochord
IHC Days 7-13 Weberian apparatus
Phenotype Details No data available
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.