ZFIN ID: ZDB-FIG-130212-23
Oksenberg et al., 2013 - Function and Regulation of AUTS2, a Gene Implicated in Autism and Human Evolution. PLoS Genetics   9(1):e1003221 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 2 auts2 48 hpf morphant phenotype.

(A) Fish injected with the 5 base-pair translational MO mismatch control have similar morphology as wild type fish. Injection of the auts2 translational MO results in fish with a stunted development phenotype that includes a smaller head, eyes, body and fins. (B) HuC-GFP fish injected with the 5 bp control MO display normal levels of developing neurons in the brain. HuC-GFP translational MO injected fish display considerably less developing neurons in the optic tectum (ot), retina (ret), and cerebellum (ce). (C) 5 bp mismatch control injected fish have little to non-observable apoptosis in the brain as observed by TUNEL staining, while translational MO injected fish display high levels of apoptosis, primarily in the midbrain (mb) and hindbrain (hb). (D) PCNA cell proliferation assay in the 5 bp MO control injected fish shows lower levels of cell proliferation in the brain compared to the translational MO injected fish. (E) Tg(mnx1:GFP) fish injected with the 5 bp MO control display normal levels of motor neurons versus the auts2 translational MO injected fish which have fewer motor neurons in the spinal cord (sc). In addition, motor neuron projections (mnp) are weaker and more perpendicular to the spinal cord. (F) Translational MO injected fish display fewer Rohon-Beard cells (arrowheads) in the spinal cord than morphants. All morphant fish are scaled to their 5 bp control counterparts.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-auts2a standard conditions Long-pec cell proliferation in forebrain increased occurrence, abnormal
Long-pec cell proliferation in hindbrain increased occurrence, abnormal
Long-pec cell proliferation in midbrain increased occurrence, abnormal
Long-pec eye decreased size, abnormal
Long-pec fin decreased size, abnormal
Long-pec head decreased size, abnormal
Long-pec spinal cord Rohon-Beard neuron decreased amount, abnormal
Long-pec trunk decreased size, abnormal
knu3Tg + MO1-auts2a standard conditions Long-pec - Protruding-mouth cerebellum neuron decreased amount, abnormal
Long-pec - Protruding-mouth optic tectum neuron decreased amount, abnormal
ml2Tg + MO1-auts2a standard conditions Long-pec motor neuron neuron projection decreased size, abnormal
Long-pec motor neuron neuron projection perpendicular to spinal cord, abnormal
Long-pec spinal cord motor neuron decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.