ZFIN Figure: Oksenberg et al., 2013, Fig. 2

ZFIN ID: ZDB-FIG-130212-23

ZFIN ID: ZDB-FIG-130212-23

Oksenberg et al., 2013 - Function and Regulation of AUTS2, a Gene Implicated in Autism and Human Evolution. PLoS Genetics 9(1):e1003221 Full text @ PLoS Genet.

ADDITIONAL FIGURES

PHENOTYPE:

Fish:	WT + MO1-auts2a knu3Tg + MO1-auts2a ml2Tg + MO1-auts2a
Knockdown Reagent:	MO1-auts2a
Observed In:	cell proliferation in forebrain cell proliferation in hindbrain cell proliferation in midbrain cerebellum neuron eye fin head motor neuron neuron projection optic tectum neuron spinal cord spinal cord motor neuron spinal cord Rohon-Beard neuron trunk
Stage Range:	Long-pec to Protruding-mouth

Fig. 2 auts2 48 hpf morphant phenotype.

(A) Fish injected with the 5 base-pair translational MO mismatch control have similar morphology as wild type fish. Injection of the auts2 translational MO results in fish with a stunted development phenotype that includes a smaller head, eyes, body and fins. (B) HuC-GFP fish injected with the 5 bp control MO display normal levels of developing neurons in the brain. HuC-GFP translational MO injected fish display considerably less developing neurons in the optic tectum (ot), retina (ret), and cerebellum (ce). (C) 5 bp mismatch control injected fish have little to non-observable apoptosis in the brain as observed by TUNEL staining, while translational MO injected fish display high levels of apoptosis, primarily in the midbrain (mb) and hindbrain (hb). (D) PCNA cell proliferation assay in the 5 bp MO control injected fish shows lower levels of cell proliferation in the brain compared to the translational MO injected fish. (E) Tg(mnx1:GFP) fish injected with the 5 bp MO control display normal levels of motor neurons versus the auts2 translational MO injected fish which have fewer motor neurons in the spinal cord (sc). In addition, motor neuron projections (mnp) are weaker and more perpendicular to the spinal cord. (F) Translational MO injected fish display fewer Rohon-Beard cells (arrowheads) in the spinal cord than morphants. All morphant fish are scaled to their 5 bp control counterparts.

Gene Expression Details No data available

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT + MO1-auts2a	standard conditions	Long-pec	cell proliferation in forebrain increased occurrence, abnormal
		Long-pec	cell proliferation in hindbrain increased occurrence, abnormal
		Long-pec	cell proliferation in midbrain increased occurrence, abnormal
		Long-pec	eye decreased size, abnormal
		Long-pec	fin decreased size, abnormal
		Long-pec	head decreased size, abnormal
		Long-pec	spinal cord Rohon-Beard neuron decreased amount, abnormal
		Long-pec	trunk decreased size, abnormal
knu3Tg + MO1-auts2a	standard conditions	Long-pec - Protruding-mouth	cerebellum neuron decreased amount, abnormal
		Long-pec - Protruding-mouth	optic tectum neuron decreased amount, abnormal
ml2Tg + MO1-auts2a	standard conditions	Long-pec	motor neuron neuron projection decreased size, abnormal
		Long-pec	motor neuron neuron projection perpendicular to spinal cord, abnormal
		Long-pec	spinal cord motor neuron decreased amount, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.