Fig. 3
- ID
- ZDB-FIG-130123-6
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- Opitz et al., 2012 - Transgenic zebrafish illuminate the dynamics of thyroid morphogenesis and its relationship to cardiovascular development
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Early thyroid morphogenesis occurs in close spatial proximity to the apical pole of the heart. ((A)-(D)) Stills from a time-lapse movie of a tg(tg:mCherry;myl7:EGFP) embryo showing that thyroid budding and relocation from the pharynx is highly coordinated with the descent of the heart. EGFP labels the myocardium and mCherry the thyroid. Live imaging was started at 38 hpf and lasted for 17 h. The timer indicates the time elapsed since the beginning of live imaging. Lateral views are shown. The dotted line facilitates visualization of the relative movement of the thyroid (arrow in (A)). ((E)-(J)) Whole-mount IF staining of tg(tg:mCherry;myl7:EGFP) embryos confirmed the intimate contact between thyroid primordium and distal ventricular myocardium during thyroid invagination from the pharynx. Panels (E)-(G) show epifluorescence images of whole mount embryos. Panels (H)-(J) show confocal images of sagittal vibratome sections of stained embryos. mCherry, EGFP and E-Cadherin label thyroid primordium, myocardium and foregut epithelium, respectively. In all panels, anterior is to the left. Scale bar: 50 μM. |
Reprinted from Developmental Biology, 372(2), Opitz, R., Maquet, E., Huisken, J., Antonica, F., Trubiroha, A., Pottier, G., Janssens, V., and Costagliola, S., Transgenic zebrafish illuminate the dynamics of thyroid morphogenesis and its relationship to cardiovascular development, 203-216, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.