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ZFIN ID: ZDB-FIG-121130-11
Hailey et al., 2012 - Loss of Slc4a1b Chloride/Bicarbonate Exchanger Function Protects Mechanosensory Hair Cells from Aminoglycoside Damage in the Zebrafish Mutant persephone. PLoS Genetics   8(10):e1002971 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5

Fig. 1 Phenotype of the persephone mutant.

(A) Hair cell protection in homozygous persephone mutants. 5 dpf zebrafish (progeny of a heterozygous incross) were treated with or without 200 μM neomycin for 30 min, and then rinsed. After 1 hr recovery in fresh embryo media, hair cells were labeled with the vital dye DASPEI. Left panels are differential interference contrast (DIC) images and right panels are corresponding fluorescent images. Top, wildtype sibling (+/+), mock-treated, Middle, wildtype sibling (+/+), treated with 200 μM neomycin, and Bottom, persephone homozygote treated with 200 μM neomycin. Red arrows indicate examples of neuromasts present in the untreated wildtype and treated persephone larvae. Mutant larvae show dramatic retention of hair cells relative to their siblings. (B) Parvalbumin antibody staining of hair cells in representative fish from an in-cross of persephone heterozygotes. Wildtype siblings show loss of hair cells when treated with 200 μM neomycin. Homozygous persephone mutants show a dramatic retention of parvalbumin-stained hair cells following neomycin treatment, consistent with DASPEI hair cell staining results. (C) Protection observed in persephone is not due to a delay in neomycin-induced hair cell death. persephone mutants (blue line) exposed to neomycin for 1 hr, rinsed and maintained for 6 or 24 hr in fresh EM, and then assessed for hair cell death, do not show significantly greater hair cell death than those assayed after 1 hr. Hair cell death is not delayed in persephone. Wildtype siblings treated in parallel (green line) are shown for comparison. (n = 10 fish, 10 neuromasts per fish; Error bars: S.D.; p value<0.001).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pvalb IHC WT control Day 5 neuromast hair cell
IHC WT chemical treatment: framycetin Day 5 neuromast
IHC slc4a1bw39/w39(AB) chemical treatment: framycetin Day 5 neuromast hair cell
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: framycetin Day 5 anterior lateral line neuromast decreased amount, normal
Day 5 mandibular arch skeleton morphology, normal
Day 5 melanophore stripe color pattern, normal
Day 5 neuromast morphology, normal
Day 5 neuromast hair cell decreased amount, normal
Day 5 neuromast hair cell morphology, normal
Day 5 swim bladder inflated, normal
slc4a1bw39/w39(AB) chemical treatment: framycetin Day 5 mandibular arch skeleton morphology, normal
Day 5 melanophore stripe color pattern, normal
Day 5 neuromast morphology, normal
Day 5 neuromast hair cell increased amount, abnormal
Day 5 neuromast hair cell morphology, normal
Day 5 swim bladder inflated, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.