Phenotype of liver-specific supv3l1 inactivation. (A–D) Confocal images of the Left (A and B) and Right (C and D) sides of 21-d-old LiPan (A and C) and LiPan/supv3l1LKO (B and D) fish showing detached hepatocytes (white arrows) (B and D) and smaller right liver lobe (white arrow) in supv3l1LKO fish (D). (E and F) Histological analysis of wild-type (E) and supv3l1LKO (F) fish indicate disorganized liver with dead cells (arrowhead) but normal intestinal lumen (black arrows) in supv3l1LKO fish (F). (F) Survival curve of supv3l1LKO fish (lens YFP+) compared with supv3l1GtNf/GtNf siblings (lens YFP-) during the first 6 wk of life. One group was fed a larval diet until 28 dpf followed by a juvenile diet (red) and the other group was kept on the larval diet (blue). (G) Photograph of a wild-type and a supv3l1LKO mutant at 5 wk of age showing the smaller size of the mutant.