ZFIN ID: ZDB-FIG-121025-6
Glover et al., 2012 - The Usher gene cadherin 23 is expressed in the zebrafish brain and a subset of retinal amacrine cells. Molecular Vision   18:2309-2322
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Day 4 to Days 14-20
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 4 to Days 14-20

Fig. 4

Retinal morphology is normal in cdh23 mutants. Zebrafish lines carrying one of two different severe cdh23 mutant alleles, which each cause profound deafness/balance defects, were examined for retinal defects. A, B: Examination of 4 days postfertilization (dpf) retinal cryosections by differential interference contrast microscopy (left panel), by staining with the nuclear dye DAPI (middle panel), or by examining GABAergic amacrine cell morphology and the inner nuclear layer banding pattern with anti-GAD65/67 (right panel) showed that cdh23tc317e siblings (A) and homozygous mutants (B) had morphologically identical retinas. C, D: Siblings and mutants of the cdh23tj264a allele were raised to 17 dpf and their retinas were examined as in A, B. No differences in morphology between siblings and mutants were observed at this later stage of development. Scale bar=50 µm in A, left panel (applies to A and B left panels). Scale bar=20 µm for middle, right panels in A, C (also applies to image directly below).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-gad IHC WT standard conditions Day 4 GABAergic neuron
IHC Day 4 retinal inner nuclear layer amacrine cell
IHC Days 14-20 GABAergic neuron
IHC Days 14-20 retinal inner nuclear layer amacrine cell
IHC cdh23tc317e/tc317e standard conditions Day 4 GABAergic neuron
IHC Day 4 retinal inner nuclear layer amacrine cell
IHC cdh23tj264/tj264 standard conditions Days 14-20 GABAergic neuron
IHC Days 14-20 retinal inner nuclear layer amacrine cell
Phenotype Details
Fish Conditions Stage Phenotype
cdh23tc317e/tc317e standard conditions Day 4 retina layer formation process quality, normal
Day 4 retina morphology, normal
cdh23tj264/tj264 standard conditions Days 14-20 retina layer formation process quality, normal
Days 14-20 retina morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Molecular Vision for permission to reproduce figures from this article. Please note that this material may be protected by copyright.