ZFIN ID: ZDB-FIG-121011-2
Seiler et al., 2012 - Smooth muscle tension induces invasive remodeling of the zebrafish intestine. PLoS Biology   10(9):e1001386 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 1 Intestinal epithelial invasion in mlt larvae.

(A–C) Live images of wild type (WT) and mlt larvae. In WT (A) the posterior intestine forms a smooth cylindrical tube (box), whereas in mlt at 74 hpf the intestinal contour is irregular (B). Cystic expansion of the intestine is evident in 86 hpf mlt larvae (C). (D–H) Histological cross-sections through the posterior intestine of larvae immunostained for laminin (green) and cytokeratin (red). The WT intestine is comprised of a simple epithelial sheet consisting of a single layer of cells, whereas in mlt epithelial stratification (asterisks) and invasive cells that have breached the basement membrane are evident (E–G arrowheads). The initial invasive behavior is followed by expansive growth and loss of epithelial architecture (H).

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-lama1 IHC WT standard conditions Protruding-mouth intestine basal lamina
IHC myh11am498/m498 standard conditions Protruding-mouth intestine basal lamina
Ab2-krt IHC WT standard conditions Protruding-mouth intestine cytoskeleton
IHC myh11am498/m498 standard conditions Protruding-mouth intestine cytoskeleton
Phenotype Details
Fish Conditions Stage Phenotype
myh11am498/m498 standard conditions Protruding-mouth intestinal epithelial cell mislocalised, abnormal
myh11am498/m498 standard conditions Protruding-mouth intestinal epithelium cystic, abnormal
myh11am498/m498 standard conditions Protruding-mouth intestinal epithelium stratified, abnormal
myh11am498/m498 standard conditions Protruding-mouth intestinal epithelium basal lamina degenerate, abnormal
myh11am498/m498 standard conditions Protruding-mouth intestinal epithelium focal adhesion degenerate, abnormal
myh11am498/m498 standard conditions Protruding-mouth intestinal epithelium invadopodium present, abnormal
myh11am498/m498 standard conditions Protruding-mouth intestine morphology, abnormal
myh11am498/m498 standard conditions Protruding-mouth regulation of intestinal epithelial structure maintenance disrupted, abnormal
myh11am498/m498; p306Tg standard conditions Protruding-mouth intestinal epithelial cell mislocalised, abnormal
Protruding-mouth intestinal epithelium cystic, abnormal
Protruding-mouth intestinal epithelium stratified, abnormal
Protruding-mouth intestinal epithelium basal lamina degenerate, abnormal
Protruding-mouth intestinal epithelium focal adhesion degenerate, abnormal
Protruding-mouth intestinal epithelium invadopodium present, abnormal
Protruding-mouth intestine morphology, abnormal
Protruding-mouth regulation of intestinal epithelial structure maintenance disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.