ZFIN ID: ZDB-FIG-120224-7
Hugo et al., 2012 - A monocarboxylate transporter required for hepatocyte secretion of ketone bodies during fasting. Genes & Development   26(3):282-93 Full text @ Genes & Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 4 Slc16a6a is a β-hydroxybutyrate transporter. (A) RT–PCR of mature slc16a6a and rpp0 transcripts at the indicated ages. (B) Whole-mount in situ hybridization with slc16a6a riboprobes. (C) The topology of the predicted wild-type and hypothetical rmn Slc16a6a proteins. (D) Ponceau S staining of nitrocellulose membranes following transfer of proteins resolved by sodium dodecyl sulfate polyacrylamide electrophoresis. (E) Immunoblotting with custom anti-Slc16a6a IgGs of the membrane in D. The membrane was deliberately overexposed in order to increase the chance of detecting a smaller protein band in the rmn mutant. (F) Current tracings of Xenopus oocytes expressing Slc16a6a (or control-injected oocytes, in black) that were perfused with monocarboxylic acids (5 mM) for the indicated time period. Oocytes were voltage-clamped to a holding potential of 70 mV. (G) Current–voltage relation of a Slc16a6a-expressing oocyte perfused with β-hydroxybutyrate.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
slc16a6a WIK standard conditions 1-cell to 1-4 somites Not Detected whole organism RTPCR
5-9 somites to Day 5 whole organism RTPCR
Prim-5 head ISH
Prim-5 pronephros ISH
Protruding-mouth head ISH
Protruding-mouth pronephros ISH
Day 5 head ISH
Day 5 intestinal bulb ISH
Day 5 liver ISH
Day 5 swim bladder ISH
Ab1-slc16a6a Adult liver WB
Ab1-slc16a6a slc16a6as951/s951; gz2Tg; gz4Tg; m1018Tg(WIK) standard conditions Adult Not Detected liver WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
slc16a6as951/s951; gz2Tg; gz4Tg; m1018Tg(WIK) standard conditions Adult monocarboxylic acid transmembrane transporter activity decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Genes & Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Genes & Dev.