ZFIN ID: ZDB-FIG-120224-7 |
Fish: | |
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Observed In: | |
Stage: | Adult |
Fig. 4 Slc16a6a is a β-hydroxybutyrate transporter. (A) RT–PCR of mature slc16a6a and rpp0 transcripts at the indicated ages. (B) Whole-mount in situ hybridization with slc16a6a riboprobes. (C) The topology of the predicted wild-type and hypothetical rmn Slc16a6a proteins. (D) Ponceau S staining of nitrocellulose membranes following transfer of proteins resolved by sodium dodecyl sulfate polyacrylamide electrophoresis. (E) Immunoblotting with custom anti-Slc16a6a IgGs of the membrane in D. The membrane was deliberately overexposed in order to increase the chance of detecting a smaller protein band in the rmn mutant. (F) Current tracings of Xenopus oocytes expressing Slc16a6a (or control-injected oocytes, in black) that were perfused with monocarboxylic acids (5 mM) for the indicated time period. Oocytes were voltage-clamped to a holding potential of 70 mV. (G) Current–voltage relation of a Slc16a6a-expressing oocyte perfused with β-hydroxybutyrate. |
Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
---|---|---|---|---|---|---|---|
slc16a6a | WIK | standard conditions | 1-cell to 1-4 somites | Not Detected | whole organism | RTPCR | |
5-9 somites to Day 5 | whole organism | RTPCR | |||||
Prim-5 | head | ISH | |||||
Prim-5 | pronephros | ISH | |||||
Protruding-mouth | head | ISH | |||||
Protruding-mouth | pronephros | ISH | |||||
Day 5 | head | ISH | |||||
Day 5 | intestinal bulb | ISH | |||||
Day 5 | liver | ISH | |||||
Day 5 | swim bladder | ISH | |||||
Ab1-slc16a6a | Adult | liver | WB | ||||
Ab1-slc16a6a | slc16a6as951/s951; gz2Tg; gz4Tg; m1018Tg(WIK) | standard conditions | Adult | Not Detected | liver | WB |
Fish | Conditions | Stage | Phenotype |
---|---|---|---|
slc16a6as951/s951; gz2Tg; gz4Tg; m1018Tg(WIK) | standard conditions | Adult | monocarboxylic acid transmembrane transporter activity decreased process quality, abnormal |
Acknowledgments: | ZFIN wishes to thank the journal Genes & Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Genes & Dev. |
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