FIGURE

Fig. S5

ID

ZDB-FIG-120216-107

Publication

Van Otterloo et al., 2012 - Novel Tfap2-mediated control of soxE expression facilitated the evolutionary emergence of the neural crest

Other Figures

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Fig. S5

Nuclear anti-Tfap2a immunoreactivity is specific for Tfap2a protein. (A-D) Lateral views of cells of the enveloping layer (EVL), covering the yolk of 24 hpf embryos fixed and processed to reveal anti-Tfap2a immunoreactivity (IR). (A,C) Wild-type and (B,D) presumed tfap2a mutant embryos that are either (A,B) uninjected or (C,D) injected with MO targeting tfap2c. (A,C) In wild-type embryos, anti-Tfap2a IR is visible in nuclei (arrows) and at cell boundaries (red asterisks). (B) Presumed tfap2a mutants lack nuclear staining. Cell boundary staining persists, suggesting that it is non-specific. In (C) wild-type embryos injected with a tfap2c MO, nuclear anti-Tfap2a IR persists, suggesting that anti-Tfap2a is specific to Tfap2a and does not recognize Tfap2c. (D) A presumed tfap2a mutant injected with tfap2c MO.

Expression Data

Antibody:	Ab1-tfap2a
Fish:	WT tfap2a^ts213/ts213 WT + MO2-tfap2c tfap2a^ts213/ts213 + MO2-tfap2c
Knockdown Reagent:	MO2-tfap2c
Anatomical Term:	epidermis nucleus
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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