ZFIN ID: ZDB-FIG-120202-6
Ulitsky et al., 2011 - Conserved Function of lincRNAs in Vertebrate Embryonic Development despite Rapid Sequence Evolution. Cell   147(7):1537-1550 Full text @ Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 6

The Importance of linc-birc6 for Proper Brain Development (A) In situ hybridization showing megamind expression in the brain and eyes of zebrafish embryos at 28 hpf. (B) Gene architecture of megamind, showing the hybridization sites of the MOs (red boxes) and RT-PCR primers (arrows). (C) Semiquantitative RT-PCR of mature megamind in embryos at 72 hpf that had been injected with the indicated MOs. β-actin mRNA was used as a control. (D) Brain ventricles after injection with either the indicated MOs or coinjected with the splice-site MO and mature mouse megamind RNA, visualized using a red fluorescent dye injected into the ventricle space at 28 hpf. An expanded midbrain ventricle (arrow) and abnormal hindbrain hinge point (asterisk) are indicated. (E) Embryos at 48 hpf that had been injected with the indicated reagents. Abnormal head shape and enlarged brain ventricles are indicated (arrow). (F) Embryos at 48 hpf that had been injected with the indicated reagents. NeuroD-positive neurons in the retina and nasal placode were marked with GFP expressed from the neurod promoter (Obholzer et al., 2008). Near absence of NeuroD-positive neurons in the retina and tectum (arrows) is indicated. (G) Frequency of morphant phenotypes in injected embryos (Table S5). (H) Schematic of DNA point substitutions in the megamind-conserved segment. (I) Architecture of a hybrid transcript containing the megamind-conserved segment in the context of cyrano-flanking sequences. See also Figure S5 and Table S5.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
birc6-as2 WT standard conditions Prim-5 brain ISH
Prim-5 eye ISH
EGFP nl1Tg standard conditions Long-pec optic tectum IFL
Long-pec retina IFL
nl1Tg + MO1-birc6-as2 + MO2-birc6-as2 standard conditions Long-pec optic tectum IFL
Long-pec retina IFL
nl1Tg + MO3-birc6-as2 standard conditions Long-pec optic tectum IFL
Long-pec retina IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-birc6-as2 + MO2-birc6-as2 standard conditions Prim-5 brain development disrupted, abnormal
Prim-5 forebrain ventricle decreased size, abnormal
Prim-5 tectal ventricle distended, abnormal
Long-pec brain development disrupted, abnormal
Long-pec eye decreased size, abnormal
Long-pec head decreased size, abnormal
Long-pec ventricular system hydrocephalic, abnormal
WT + MO3-birc6-as2 standard conditions Prim-5 brain development disrupted, abnormal
Prim-5 forebrain ventricle decreased size, abnormal
Prim-5 tectal ventricle distended, abnormal
Long-pec eye decreased size, abnormal
Long-pec head decreased size, abnormal
Long-pec ventricular system hydrocephalic, abnormal
nl1Tg + MO1-birc6-as2 + MO2-birc6-as2 standard conditions Long-pec optic tectum EGFP expression decreased distribution, abnormal
Long-pec retina EGFP expression decreased distribution, abnormal
nl1Tg + MO3-birc6-as2 standard conditions Long-pec optic tectum EGFP expression decreased distribution, abnormal
Long-pec retina EGFP expression decreased distribution, abnormal
Acknowledgments:
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Reprinted from Cell, 147(7), Ulitsky, I., Shkumatava, A., Jan, C.H., Sive, H., and Bartel, D.P., Conserved Function of lincRNAs in Vertebrate Embryonic Development despite Rapid Sequence Evolution, 1537-1550, Copyright (2011) with permission from Elsevier. Full text @ Cell