FIGURE

Fig. S3

ID

ZDB-FIG-120127-16

Publication

Wong et al., 2012 - Hedgehog signaling is required for differentiation of endocardial progenitors in zebrafish

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Fig. S3

Reduced endocardium is present in CyA-treated embryos at 48hpf while NFATc1 expression is absent.

(A,B) Endocardium and myocardium visualized by etsrp:GFP (green) and cmlc2:mCherry (red) expression in live DMSO control (A) and CyA-treated embryos (B). Note the presence of reduced myocardium and endocardium and the absence of heart looping in CyA-treated embryos. (C,D) Endocardial NFATc1 expression is absent in CyAtreated embryos (D) as compared to DMSO-treated controls (C). All panels, ventral view of whole-mounted embryos, anterior is to the top. (A,B) Maximal projection images were obtained using compound fluorescence microscopy (Zeiss, AxioImager) and subjected to 3Ddeconvolution (AutoQuant software package).

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 361(2), Wong, K.S., Rehn, K., Palencia-Desai, S., Kohli, V., Hunter, W., Uhl, J.D., Rost, M.S., and Sumanas, S., Hedgehog signaling is required for differentiation of endocardial progenitors in zebrafish, 377-91, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.