|ZFIN ID: ZDB-FIG-111201-45|
Fig. 2 Expression of Nsf in the CNS and lateral line organ (5 dpf).
A, An antibody against human NSF protein labels the larval nervous system (5 dpf). B, Labeling is absent in nsfst53 mutants. Images are projections of 5 optical sections of 10 μm thickness. Scale bar, 100 μm. C-F, Confocal projections (10×1 μm sections) of anti-NSF (red) and Ribeye b (Blue) in TgBAC(neurod:EGFP)nl1 transgenic fish. Nsf signal colocalized with anti-GFP (green) in the afferent nerve, and was also in Ribeye b-positive hair cells. Scale bar, 10 μm.
|EGFP||nl1Tg||standard conditions||Day 5||lateral line nerve||IFL|
|Ab1-nsf||IHC||WT||standard conditions||Day 5||nervous system|
|IHC||nl1Tg||standard conditions||Day 5||lateral line nerve|
|IHC||Day 5||neuromast hair cell|
|IHC||nsfast53/st53||standard conditions||Day 5||Not Detected||nervous system|
|Ab3-ctbp2l||IHC||nl1Tg||standard conditions||Day 5||neuromast hair cell|
|Acknowledgments:||ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One|