ZFIN ID: ZDB-FIG-111007-17
Kim et al., 2011 - Cug2 is essential for normal mitotic control and CNS development in zebrafish. BMC Developmental Biology   11(1):49 Full text @ BMC Dev. Biol.
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 4 cug2 deficiency causes neurodegeneration in developing embryos. A, B. In huC:GFP transgenic embryos, injection of cug2 MO (B) causes a reduction in the number of neurons in the nucleus of the medial longitudinal fasciculus (nMLF, arrows) and the rhombomere (white spots) compared to control MO (A). Scale bar = 200 μm. C, D. Anti-acetylated α-tubulin staining of the brain of control (C) and cug2 morphants (D). cug2 deficiency causes axonal scaffolding defects in the anterior commissure (ac), olfactory nerve (ofn), nMLF (arrows), and hindbrain commissure (white spots) at 26 hpf. Scale bar = 200 μm. E, F. Anti-acetylated α-tubulin staining of the spinal cord of cug2 morphants (F) at 26 hpf shows reduced arborization in Rohon-Beard (RB) sensory neurons compared to control (E). Scale bar = 100 μm. G-J. Histological sections of control (G, I) and cug2 MO (H, J)-injected embryos at 3 dpf. cug2 morphants exhibit severely disrupted retina layer formation (H) and a much smaller neural tube that contains fewer cells (J). gcl, ganglion cell layer; inl, inner nuclear layer; ipl, inner plexiform layer; le, lens; n, notochord; on, optic nerve; opl, outer plexiform layer; pcl, photoreceptor cell layer; pe, pigmented epithelium. Scale bars = 50 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-cenpw standard conditions Protruding-mouth neural tube cell decreased amount, abnormal
Protruding-mouth neural tube decreased size, abnormal
Protruding-mouth retina layer formation disrupted, abnormal
knu3Tg + MO2-cenpw standard conditions Prim-5 anterior commissure axon organization quality, abnormal
Prim-5 medial longitudinal fasciculus neuron decreased amount, abnormal
Prim-5 rhombomere neuron decreased amount, abnormal
Prim-5 Rohon-Beard neuron decreased branchiness, abnormal
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.