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ZFIN ID: ZDB-FIG-110920-74
Eames et al., 2011 - Mutations in fam20b and xylt1 Reveal That Cartilage Matrix Controls Timing of Endochondral Ossification by Inhibiting Chondrocyte Maturation. PLoS Genetics   7(8):e1002246 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 6

fam20b mutants display reduced proteoglycan immunoreactivity

A–F, Ventral views of confocal images of embryonic heads immunostained for chondroitin sulfate proteoglycan (CSPG) at 3 dpf (A,B) or heparan sulfate PG (HSPG) at 4 dpf (C–F). Compared to abundant CSPGs detected in wild-type skeletal elements (A), fam20bb1127 mutants showed decreased CSPG immunoreactivity (B). HSPGs were abundant in gill filaments and ventral muscle groups of both wild-type (C) and fam20bb1127 mutant zebrafish (D). HSPG immunoreactivity was reduced greatly around developing chondrocytes of the fam20bb1127 mutant ceratohyal (outline in F), compared to those of wild-type siblings (outline in E). Also, the number of intracellular HSPG aggregates (arrowhead) was increased in fam20bb1127 mutant chondrocytes. Abbreviations: A = anterior; αCS = anti-chondroitin sulfate; αHS = anti-heparan sulfate; c = chondrocyte; ch = ceratohyal; e = eye; gf = gill filament; ih = interhyoideus; imp = intermandibularis posterior; Mk = Meckel′s; op = opercle; P = posterior; pq = palatoquadrate.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-10E4 IHC WT standard conditions Day 4 gill filament
IHC Day 4 interhyoideus
IHC Day 4 ventral intermandibularis posterior
IHC fam20bb1127/b1127(AB) standard conditions Day 4 gill filament
IHC Day 4 interhyoideus
IHC Day 4 ventral intermandibularis posterior
Ab-CS-56 IHC WT standard conditions Protruding-mouth ceratohyal cartilage
IHC Protruding-mouth Meckel's cartilage
IHC Protruding-mouth opercle
IHC Protruding-mouth palatoquadrate cartilage
IHC fam20bb1127/b1127(AB) standard conditions Protruding-mouth ceratohyal cartilage
IHC Protruding-mouth Meckel's cartilage
IHC Protruding-mouth opercle
IHC Protruding-mouth palatoquadrate cartilage
Phenotype Details
Fish Conditions Stage Phenotype
fam20bb1127/b1127(AB) standard conditions Day 4 ceratohyal cartilage chondrocyte morphology, abnormal
Day 4 proteoglycan biosynthetic process disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.