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Fig. 4

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ZDB-FIG-110720-72

Publication

Xiao et al., 2011 - Assembly of Lamina-Specific Neuronal Connections by Slit Bound to Type IV Collagen

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Fig. 4

Collagen IV Interacts Genetically with, and Directly Binds to, Slit(A and B) Projections of confocal images of BGUG-labeled RGC axons in a WT (A) and a double-heterozygous carrier of drg^s510 and ast^ti272z (B) at 6 dpf. Arrows point at axons that trespass the laminae.(C) Quantification of RGC axon targeting defects.(D–F) Analysis of Col IV, Slit1 and Agrin interactions using solid-phase ELISA binding assays (n = 3, with duplicate samples in each experiment). Agrin and Slit1 bind Col IV via protein-protein interactions (HS independent, not inhibited by exogenous heparin), and Agrin binds Slit1 via its HS-GAG chains (marked reduction in presence of heparin).(G) Analysis of Col IV ligand binding to Slit1 using surface plasmon resonance. Col IV binds to Slit1 in a dose-dependent fashion, with K_D = 0.65 nM.Error bars represent the SEM. See also Figure S3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 146(1), Xiao, T., Staub, W., Robles, E., Gosse, N.J., Cole, G.J., and Baier, H., Assembly of Lamina-Specific Neuronal Connections by Slit Bound to Type IV Collagen, 164-176, Copyright (2011) with permission from Elsevier. Full text @ Cell