Fig. 6

Analysis of secretory cell differentiation along anterior-posterior axis of intestines in klf4a morphants.

102-hpf wild type and klf4a morphant embryos were hybridized with glucagon RNA probe and processed for paraffin embedding and sectioning. Paraffin sections were then used for alcain blue and hematoxylin staining. (A) A section shows complete intestine including intestinal bulb (IB)/foregut, mid-intestine/midgut and posterior intestine/hindgut of a wild type embryo. Corresponding enlarged images of foregut, midgut, and hindgut of both wild type and klf4a-MO1 and klf4a-MO2-injected embryos are shown. Arrows indicate glucagon-expressing enteroendocrine cells and arrowheads indicate alcian blue-stained goblet cells. (B) Comparison of the percentages of goblet cell and glucagon-expressing enteroendocrine cell numbers within different segments of intestines in 102-hpf wild type and klf4a-MO1 and klf4a-MO2-injected embryos. Error bars indicate the standard error. Student′s t-test was conducted to compare klf4a morphants with wild type embryos. A, anus; IB, intestinal bulb.