FIGURE

Fig. 4

ID

ZDB-FIG-110516-55

Publication

Chauvigné et al., 2011 - Design and characterization of genetically engineered zebrafish aquaporin-3 mutants highly permeable to the cryoprotectant ethylene glycol

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Fig. 4

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Fig. 4

Functional characterization of DrAqp3b mutants. (A) P_fof control oocytes (water-injected) and oocytes expressing 1 ng cRNA encoding wild-type DrAqp3b (DrAqp3b-WT) or different DrAqp3b mutants at different pH. Values are the mean ± SEM of three experiments (n = 8-10 oocytes per construct). The asterisks denote significant differences between WT and mutant DrAqp3b at a given pH (Student′s t test, p < 0.05). (B) Immunofluorescence microscopy of water-injected oocytes (control), or oocytes expressing DrAqp3b-WT, -H53A, -H53A/G54H, -T85A and -H53A/G54H/T85A using an affinity purified anti-DrAqp3b antiserum. The arrowhead points to the oocyte plasma membrane. (C) Immunoblot of total membrane protein extracts of control oocytes or oocytes expressing DrAqp3b-WT treated or not with N-glycosidase F (PNGase F). The arrows indicate glycosylated and deglycosylated forms of DrAqp3b-WT. (D) Representative immunoblot of plasma membrane protein extracts of oocytes expressing increasing amounts of cRNA (1-8 ng) encoding DrAqp3b-WT, -T85A and -H53A/G54H/T85A treated with PNGaseF.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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