FIGURE

Fig. 5

ID

ZDB-FIG-110318-5

Publication

Morrissey et al., 2011 - PRE-1, a cis element sufficient to enhance cone- and rod- specific expression in differentiating zebrafish photoreceptors

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Fig. 5

PRE-1 is sufficient to enhance reporter expression from photoreceptor-specific promoters. A. Schematic of the promoter-reporter regions of chimeric constructs. Three copies of PRE-1 (red boxes) are sufficient to enhance expression from both cone and rod promoter fragments, whereas three copies of PRE-1-rho (navy boxes) are unable to enhance expression from the cone promoter fragment. Blue and purple rectangles represent zebrafish UV opsin and rhodopsin promoter fragments respectively, upstream of EGFP. B-E. Confocal micrographs of retinal sections from 5 dpf embryos injected with 1.2 kb ZOP-EGFP or (3x PRE-1)-1.2 kb ZOP-EGFP constructs and stained with 4C12 and zpr-1 antibodies. EGFP-positive cells co localize (arrows) with rod photoreceptors (4C12 antibody), but not with cone photoreceptors (zpr-1 antibody). Nuclei are counter-stained with DAPI.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.