ZFIN ID: ZDB-FIG-110214-58
Maurya et al., 2011 - Integration of Hedgehog and BMP signalling by the engrailed2a gene in the zebrafish myotome. Development (Cambridge, England)   138(4):755-765 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 3 Inhibition of Smad activity de-represses engrailed expression. (A,B) Parasagittal optical sections of 22 ss embryos showing distribution of Eng (4D9) and pSmad in wild type (A) and bmpr1ba morphants (B). Somites shown are at the level of the yolk sac. (C,D,G) Attenuation of BMPR1ba causes ectopic expression of eng2a. The histogram in G compares eGFP expression in Tg(eng2a:eGFP)i233 embryos injected with bmpr1ba morpholino, with that in non-injected controls; bars represent the frequency of eGFP expressing slow fibres at different dorsoventral positions. Positions 8-13 represents the horizontal myoseptum. Morphants display a wide expansion of the eGFP domain. Data based on three consecutive somites at the end of yolk extension in 22 ss embryos. x axis, dorsoventral position; y axis, number of eGFP+ fibres. Error bars indicate s.d. Representative examples are shown in C,D. (E) Parasagittal optical sections of a 24 hour Tg(eng2a:eGFP)i233;disp1tf18b mutant embryo displaying reduced slow fibres (F59; red) and near absence of eng2a:eGFP. (F) Similarly staged Tg(eng2a:eGFP)i233; disp1tf18b mutant embryo that was injected with bmpr1ba morpholino at the one- to two-cell stage, showing robust rescue of eGFP expression. (H) Parasagittal optical sections of a 24 hour Tg(eng2a:eGFP)i233; smob641 mutant embryo injected with bmpr1ba morpholino at one- to two-cell stage, showing complete absence of eng2a:eGFP expression. Inhibition of Smad activity de-represses engrailed expression. (A,B) Parasagittal optical sections of 22 ss embryos showing distribution of Eng (4D9) and pSmad in wild type (A) and bmpr1ba morphants (B). Somites shown are at the level of the yolk sac. (C,D,G) Attenuation of BMPR1ba causes ectopic expression of eng2a. The histogram in G compares eGFP expression in Tg(eng2a:eGFP)i233 embryos injected with bmpr1ba morpholino, with that in non-injected controls; bars represent the frequency of eGFP expressing slow fibres at different dorsoventral positions. Positions 8-13 represents the horizontal myoseptum. Morphants display a wide expansion of the eGFP domain. Data based on three consecutive somites at the end of yolk extension in 22 ss embryos. x axis, dorsoventral position; y axis, number of eGFP+ fibres. Error bars indicate s.d. Representative examples are shown in C,D. (E) Parasagittal optical sections of a 24 hour Tg(eng2a:eGFP)i233;disp1tf18b mutant embryo displaying reduced slow fibres (F59; red) and near absence of eng2a:eGFP. (F) Similarly staged Tg(eng2a:eGFP)i233; disp1tf18b mutant embryo that was injected with bmpr1ba morpholino at the one- to two-cell stage, showing robust rescue of eGFP expression. (H) Parasagittal optical sections of a 24 hour Tg(eng2a:eGFP)i233; smob641 mutant embryo injected with bmpr1ba morpholino at one- to two-cell stage, showing complete absence of eng2a:eGFP expression.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP disp1tf18b/tf18b; i233Tg standard conditions Prim-5 slow muscle cell IFL
disp1tf18b/tf18b; i233Tg + MO2-bmpr1ba standard conditions Prim-5 slow muscle cell IFL
i233Tg + MO2-bmpr1ba standard conditions 20-25 somites slow muscle cell IFL
smob641/b641; i233Tg + MO2-bmpr1ba standard conditions Prim-5 Not Detected slow muscle cell IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC disp1tf18b/tf18b; i233Tg standard conditions Prim-5 slow muscle cell
IHC disp1tf18b/tf18b; i233Tg + MO2-bmpr1ba standard conditions Prim-5 slow muscle cell
IHC i233Tg + MO2-bmpr1ba standard conditions 20-25 somites slow muscle cell
Ab1-eng IHC WT standard conditions 20-25 somites slow muscle cell
IHC WT + MO2-bmpr1ba standard conditions 20-25 somites slow muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-bmpr1ba standard conditions 20-25 somites myotome cellular quality, abnormal
20-25 somites skeletal muscle cell differentiated, abnormal
disp1tf18b/tf18b; i233Tg standard conditions Prim-5 slow muscle cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development