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ZIRC
ZFIN ID: ZDB-FIG-101223-8
Diz-Munoz et al., 2010 - Control of Directed Cell Migration In Vivo by Membrane-to-Cortex Attachment. PLoS Biology   8(11):e1000544 Full text @ PLoS Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 75%-epiboly

Fig. 2 ERM proteins modulate prechordal plate progenitor cell protrusion formation.

(A) Examples of isolated control and ERM-deficient prechordal plate progenitor cells. (B) Maximum bleb size in isolated control and ERM-deficient prechordal plate progenitor cells. (C) Frequency of bleb formation in isolated control and ERM-deficient prechordal plate progenitor cells. (D) Animal view of the leading edge of a wt prechordal plate. Inset shows a schematic animal view of an embryo at 80% epiboly, with the green rectangle marking the imaged area in (D). Examples of a bleb, filopodium, and lamellipodium in prechordal plate leading edge cells. Arrowheads point to the protrusions. Arrow indicates the separation between actin cortex and membrane in the bleb. (E) Percentage of blebs, filopodia, and lamellipodia in wt prechordal plate leading edge cells (mean ± half standard deviation). (F) Example blebs (arrowheads) in wt and ERM-deficient prechordal plate leading edge cells. (G) Maximum bleb size in wt and ERM-deficient prechordal plate leading edge cells. (H) Frequency of bleb formation in wt and ERM-deficient prechordal plate leading edge cells. Plasma membrane (GPI-RFP) is red; actin cortex (Lifeact-GFP) is green. Scale bars = 10 μm. The projected bleb size in (B) and (G) was normalized to the projected cell size. Number of analyzed blebs in (B) and (C) = 39 (control), 42 (DNEzrin), and 51 (ezrin-MO) and in (G) = 14 (wt), 16 (DNEzrin), and 15 (ezrin-MO). Number of analyzed cells in (E) and (H) = 23 (wt), 30 (DNEzrin), and 12 (ezrin-MO). Statistical significance was determined using t test (G) or Mann–Whitney U test (B, C, and H).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-ezrb standard conditions 75%-epiboly anterior axial hypoblast bleb increased amount, abnormal
75%-epiboly anterior axial hypoblast bleb increased size, abnormal
75%-epiboly anterior axial hypoblast filopodium decreased amount, abnormal
75%-epiboly anterior axial hypoblast lamellipodium decreased amount, abnormal
75%-epiboly cytoskeletal anchoring at plasma membrane decreased process quality, abnormal
75%-epiboly regulation of cell projection assembly process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.