FIGURE

Fig. S1

ID

ZDB-FIG-101119-19

Publication

Chen et al., 2009 - Abcb10 physically interacts with mitoferrin-1 (Slc25a37) to enhance its stability and function in the erythroid mitochondria

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Fig. S1

BT-Mfrn1 properly targets to the mitochondrial compartment and complements anemia of frs mutants, and so it is validated as a functional bait for affinity purification. (A) Mouse Mfrn1 was FLAG-tagged at its N terminus as bait for affinity purification. The FLAG-tagged construct was referred as BT-Mfrn1. (B) Immunolocalization of BT-Mfrn1 protein to mitochondria of transfected COS7 cells. Fluorescence confocal images were obtained from immunostained resident mitochondrial proteins (red) and BT-Mfrn1 (green). Colocalized expression of BT-Mfrn1 in the mitochondria is indicated by the yellow signal. (C) BT-Mfrn1 proteins properly targeted to the mitochondrial fraction. Mitochondrial and cytosolic fractions were isolated from BT-Mfrn1-transfected COS7 cells and immunoblotted by anti-Mfrn1 and anti-FLAG antibodies, respectively. (D) Expression of BT-Mfrn1 cRNA rescued the anemia of frascati ( frst^q223) embryos. Control wild-type (wt), frs mutant (mt), and rescued frs embryos (r1–3) were stained with o-dianisidine to detect hemoglobinized cells. Control wild-type, mutant, heterozygote, and rescued frs (r1–3) embryos were genotyped. Genotyping results in the Right Lower confirmed that these three putative rescued embryos (r1–3) are mutants.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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