ZFIN ID: ZDB-FIG-101102-7
Bagnat et al., 2010 - Cse1l Is a Negative Regulator of CFTR-Dependent Fluid Secretion. Current biology : CB   20(20):1840-1845 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. S1 Characterization of baoss866 Mutant Phenotype (Related to Figures 1 and 3)

(A) baoss866 mutant enterocytes retain absorptive cell marker expression and localization. 120 hpf WT and baoss866 mutant larvae were fixed in PFA, sectioned and stained for the absorptive marker 4e8. In baoss866 mutant enterocytes, 4e8 (green) was expressed and localized apically as in WT. The arrow points to the apical membrane. Scale bars represent 50 μm.

(B) Delaminating cells in baoss866 mutants undergo apoptosis. 120 hpf WT and baoss866 mutant larvae were fixed in PFA, mounted, sectioned and subjected to the Tunel assay to detect apoptotic cells. While all delaminating cells in baoss866 mutant larvae were Tunel positive (red channel, arrows), the proportion of gut cells undergoing apoptosis in WT and baoss866 mutant larvae were essentially the same. Scale bars represent 25 μm.

(C) Exocrine pancreas degeneration and liver growth arrest in baoss866 mutant larvae. To visualize endodermal organs, we crossed the baoss866 mutation to a transgenic line expressing GFP and DsRed in the exocrine pancreas and liver, respectively. baoss866 mutant larvae showed a fully penetrant (~100%) exocrine pancreas phenotype (arrows) at 120 hpf. We also detected a variable degree of liver growth arrest (arrowheads) compared to WT siblings. In contrast, we did not detect any obvious pancreatic islet defects (not shown). These phenotypes are fully consistent with cse1l’s expression pattern (see Figure 3F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP cse1ls866/s866; gz15Tg standard conditions Day 5 exocrine pancreas IHC
gz15Tg standard conditions Day 5 exocrine pancreas IHC
RFP cse1ls866/s866; gz15Tg standard conditions Day 5 liver IHC
gz15Tg standard conditions Day 5 liver IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-4E8 IHC WT standard conditions Day 5 enterocyte apical part of cell
IHC cse1ls866/s866 standard conditions Day 5 enterocyte apical part of cell
Phenotype Details
Fish Conditions Stage Phenotype
cse1ls866/s866 standard conditions Day 5 digestive tract development process quality, abnormal
Day 5 gut epithelium flattened, abnormal
Day 5 gut swollen, abnormal
Day 5 intestinal villus flattened, abnormal
cse1ls866/s866; gz15Tg standard conditions Day 5 exocrine pancreas degenerate, abnormal
Day 5 exocrine pancreas development process quality, abnormal
Day 5 liver decreased size, abnormal
Day 5 liver development arrested, abnormal
Day 5 liver immature, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.