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ZFIN ID: ZDB-FIG-100809-35
Xie et al., 2010 - A novel transgenic zebrafish model for blood-brain and blood-retinal barrier development. BMC Developmental Biology   10:76 Full text @ BMC Dev. Biol.
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. S6 Morpholino knockdown of claudin 5a in zebrafish embryos results in BRB breakdown. Cldn-5a was knocked down by injecting morpholino-cldn5a into double transgenic l-fabp:DBP-EGFP;flk1:mCherry embryos at the 2-cell stage. At 4 dpf, leakage of DBP-EGFP(green) from the hyaloid vasculature (red) was observed. A 5 bp-mismatched Morpholino was injected as control and showed an intact BRB.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lri500Tg; s896Tg + MO2-cldn5a standard conditions Day 4 establishment of endothelial barrier disrupted, abnormal
Day 4 hyaloid vessel broken, abnormal
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.