ZFIN ID: ZDB-FIG-100802-5
Zhao et al., 2010 - Klf6/copeb is required for hepatic outgrowth in zebrafish and for hepatocyte specification in mouse ES cells. Developmental Biology   344(1):79-93 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Day 4

Fig. 2 copeb is required for embryonic development. Live copeb splice donor morphants and copeb ATG morphants were examined on 1–4 dpf. (a) Live images of Tg(fabp10-RFP) embryos showed copeb slice donor morphants exhibit pericardial, yolk sac and cephalic edema at 48 and 72 hpf and a small for size liver. All images are taken at the same magnification; scale bar = 100 μm. (b) Live images of embryos showed a range of phenotypes of copeb ATG morpholino-injected embryos: normal (resembling embryos injected with the control morpholino (top), moderately affected embryos with pericardial edema, unconsumed yolk (Y) and blood congestion (middle 2 embryos) and severely affected, runted embryos (bottom). Bottom panels show the pericardial edema (PE; black circle) in morphants. These characteristics were observed in at least 10 clutches of copeb ATG morphants. (c) Quantification of the percent of embryos displaying the morphant phenotype at 72 and 96 hpf demonstrate that the phenotype becomes more severe as a function of morpholino concentration and time. Values represent the average of four clutches of at least 100 embryos were scored for each clutch on each day. Error bars indicate the standard deviation of the mean. (d) copeb splice donor morphants were quantified by phenotype and compared to uninjected controls at 96 hpf. Data are the average of at least 50 embryos per group in 3 clutches. Error bars indicate the standard deviation of the mean.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gz15Tg + MO3-klf6a standard conditions Protruding-mouth blood circulation disrupted, abnormal
Protruding-mouth eye decreased size, abnormal
Protruding-mouth liver decreased size, abnormal
Protruding-mouth mandibular arch skeleton decreased size, abnormal
Protruding-mouth pericardium edematous, abnormal
Protruding-mouth whole organism decreased size, abnormal
Day 4 blood circulation disrupted, abnormal
Day 4 eye decreased size, abnormal
Day 4 gut decreased size, abnormal
Day 4 liver decreased size, abnormal
Day 4 mandibular arch skeleton decreased size, abnormal
Day 4 pericardium edematous, abnormal
Day 4 whole organism decreased size, abnormal
gz15Tg + MO4-klf6a standard conditions Prim-5 eye decreased size, abnormal
Prim-5 head decreased size, abnormal
Long-pec head edematous, abnormal
Long-pec liver decreased size, abnormal
Long-pec pericardium edematous, abnormal
Long-pec yolk edematous, abnormal
Protruding-mouth head edematous, abnormal
Protruding-mouth liver decreased size, abnormal
Protruding-mouth pericardium edematous, abnormal
Protruding-mouth yolk edematous, abnormal
Day 4 liver decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 344(1), Zhao, X., Monson, C., Gao, C., Gouon-Evans, V., Matsumoto, N., Sadler, K.C., and Friedman, S.L., Klf6/copeb is required for hepatic outgrowth in zebrafish and for hepatocyte specification in mouse ES cells, 79-93, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.