ZFIN ID: ZDB-FIG-100323-5
Chablais et al., 2010 - IGF signaling between blastema and wound epidermis is required for fin regeneration. Development (Cambridge, England)   137(6):871-879 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Adult

Fig. 3 Morpholino-mediated igf2b and igf1rab knockdowns impair fin regeneration. (A-E) Fins microinjected into their dorsal half with control (A), igf2b (B), igf1ra + igf1rb (C), igf1ra (D) and igf1rb (E) MOs. The remaining non-injected half of the fin served as an internal control to assess the normal growth rate. The left panels show fins shortly after injection and the right panels show the same fins at 48 hpi. The control MO does not affect the size of the outgrowth relative to the uninjected side (A). The specific MOs impair regeneration, leading to an asymmetric shape of the regenerate (B-E). (F) Bar chart comparing the surface of regenerates that regrew within 24 and 48 hours after MO injection in relation to the uninjected fin regenerate. The injected:uninjected regenerate surface is significantly reduced by delivery of specific MOs. n=10 fins per group; *P<0.01.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-igf1ra physical alteration: anatomical structure Adult fin regeneration arrested, abnormal
Adult fin regeneration decreased process quality, abnormal
WT + MO1-igf1ra + MO1-igf1rb physical alteration: anatomical structure Adult fin regeneration arrested, abnormal
Adult fin regeneration decreased process quality, abnormal
WT + MO1-igf1rb physical alteration: anatomical structure Adult fin regeneration arrested, abnormal
Adult fin regeneration decreased process quality, abnormal
WT + MO4-igf2b physical alteration: anatomical structure Adult fin regeneration arrested, abnormal
Adult fin regeneration decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development