ZFIN ID: ZDB-FIG-100319-22
Ko et al., 2010 - Broad-Minded Links Cell Cycle-Related Kinase to Cilia Assembly and Hedgehog Signal Transduction. Developmental Cell   18(2):237-247 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 7 CCRK Interacts with Bromi and Acts in Vertebrate Ciliogenesis
(A) HA-tagged Bromi or HA-tagged CCRK, expressed in NIH 3T3 fibroblasts by Rheoswitch ligand (RSL1)-mediated induction, coimmunoprecipitated endogenous CCRK and Bromi, respectively.
(B) CCRK protein levels in WT versus bromi mutant embryos and embryonic fibroblasts and in uninduced versus RSL1-induced Bromi-HA-expressing NIH 3T3 cells. RSL1 treatment of parental Rheoswitch cells had no effect on CCRK levels (data not shown). Anti-β-tubulin was used as a loading control.
(C) Injection of MOs (4 ng) directed against the start site (ccrk-AUG) and exon 3/intron 3 splice site (ccrk-e3i3) of the zebrafish ccrk homolog resulted in kinking of the body axis and abnormal tail curvature.
(D) Confocal microscopy of acetylated α-tubulin-stained cilia in the posterior (distal early/late) regions of the kidney tubules.
(E) Percentage of animals exhibiting curled cilia. Injection of low doses of bromi-AUG or ccrk-e3i3 MOs (1 ng or 2 ng, respectively) had little effect on their own, whereas coinjection of both MOs at these doses had a synergistic effect. Values in parentheses represent numbers of animals scored.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC WT control Long-pec pronephric tubule cilium
IHC WT + MO1-cdk20 standard conditions Long-pec pronephric tubule cilium
IHC WT + MO2-cdk20 standard conditions Long-pec pronephric tubule cilium
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cdk20 standard conditions Protruding-mouth post-vent region increased curvature, abnormal
Protruding-mouth renal tubule cilium curled, abnormal
Protruding-mouth trunk kinked, abnormal
WT + MO1-tbc1d32 + MO2-cdk20 standard conditions Long-pec renal tubule cilium curled, abnormal
WT + MO2-cdk20 standard conditions Protruding-mouth post-vent region increased curvature, abnormal
Protruding-mouth renal tubule cilium curled, abnormal
Protruding-mouth trunk kinked, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 18(2), Ko, H.W., Norman, R.X., Tran, J., Fuller, K.P., Fukuda, M., and Eggenschwiler, J.T., Broad-Minded Links Cell Cycle-Related Kinase to Cilia Assembly and Hedgehog Signal Transduction, 237-247, Copyright (2010) with permission from Elsevier. Full text @ Dev. Cell