FIGURE

Fig. 4

ID
ZDB-FIG-100223-42
Publication
Raeker et al., 2010 - Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Skeletal muscle phenotype in obscurin A RhoGEF morphant embryos at 72 hpf. Ultrastructural analysis was performed at 72 hpf on control, obscurin A (ObscMO) and obscurin A RhoGEF (ObscRhoMO) morphant embryos. In control embryos, the myofibrils are aligned in register across the sarcoplasm (A) with well defined M bands and finely “stitched” Z disks (D). In the obscurin morphant embryos (B) there is marked disturbance of myofibril organization with areas of misalignment of the M bands of adjacent myofibrils (E: ^). By comparison, the obscurin A RhoGEF morphants display myofibril organization (C) similar to control embryos but reduced myofibrillar content with more sarcoplasm devoid of myofibrils and more rounded nuclei (C, G: ^). There were also mild sarcomeric abnormalities including, infrequently, an indistinct appearance of the Z disk and absence of the M band (F: ∗) which could occur even within myofibrils demonstrating mature Z disks and M bands (F: ^). Note that in skeletal myocytes stained with DAPI, the nuclei in the obscurin A RhoGEF morphants (RhoMO) are closer together and more rounded (G) than in controls (H) consistent with reduced myofibril volume. Scale bars 2 μm (A–C), 0.5 μm (D–F) and 10 μm (G, H).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 337(2), Raeker, M.O., Bieniek, A.N., Ryan, A.S., Tsai, H.J., Zahn, K.M., and Russell, M.W., Targeted deletion of the zebrafish obscurin A RhoGEF domain affects heart, skeletal muscle and brain development, 432-443, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.