ZFIN ID: ZDB-FIG-091016-45
Norden et al., 2009 - Actomyosin is the main driver of interkinetic nuclear migration in the retina. Cell   138(6):1195-1208 Full text @ Cell
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-5

Fig. 7 IKNM Movements Are Inhibited when MyosinII Activity Is Blocked, but the Effect Can be Rescued by MRLC2T18DS19D

(A) Images from Movies S13–S15 at 3 hr intervals. Pseudocolored nuclei in WT (upper panel) travel further than nuclei treated with 100 μM Blebbistatin or 25 mM BDM (lower panels). Arrows mark a mitotic but not separated nucleus in BDM treatment.

(B) Stochastic motion velocity distributions for Blebbistatin- and BDM-treated embryos, and centrin morphants and DNp150-expressing cells treated with BDM.

(C) Stochastic motion MSD profiles (error bars show 95% confidence intervals) with linear fit for Blebbistatin- and BDM-treated embryos, and centrin morphant and DNp150-expressing cells treated with BDM.

(D) Stochastic motion velocity distributions for BDM-treated embryos injected with MRLC2T18DS19D-GFP or wild-type MRLC2-GFP, respectively.

(E) Stochastic motion MSD profiles (error bars show 95% confidence intervals) with linear fit for BDM-treated embryos injected with MRLC2T18DS19D-GFP or MRLC2-GFP, respectively. Only the activated MRLC partially rescues the effect of BDM treatment.

Scale bars represent 10 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: diacetylmonoxime Prim-5 interkinetic nuclear migration disrupted, abnormal
Prim-5 presumptive neural retina actin cytoskeleton disassembled, abnormal
Acknowledgments:
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Reprinted from Cell, 138(6), Norden, C., Young, S., Link, B.A., and Harris, W.A., Actomyosin is the main driver of interkinetic nuclear migration in the retina, 1195-1208, Copyright (2009) with permission from Elsevier. Full text @ Cell