Fig. 9

The neuroepithelium remains intact in pcdh19 morphants. Shown are images of 22 hpf embryos immunostained with antibodies against the apical epithelial marker, ZO-1. (A) Maximum intensity projection of the midbrain–hindbrain region of a wild type embryo (50 sections, spanning 100 μm). ZO-1 labels the apical surface of the neuroepithelium, which lines the midbrain and hindbrain ventricles. (B) In contrast to the ZO-1 labeling in wild type embryos, much of the apical face of the neuroepithelium in morphant embryos is oriented dorsally, more parallel to the surface of the embryo. (C) A YZ-plane through the area indicated in A, highlighting that the apical–basal axis of the neuroepithelium is aligned with the mediolateral axis of the embryo. (D) A YZ-plane through the area indicated in B. In contrast to wild type embryos, much of the apico-basal axis of the neuroepithelium remains aligned with the dorsoventral axis in morphant embryos.