Fig. 3

Analysis of zebrafish foxg1b expression by in situ hybridization. (A) Expression in the anterior neural plate at 10 hpf. (B) Expression is mainly restricted to the eye primordia at 16 hpf. (C) Dorsal view of the same embryo as in (B) at higher magnification. At 1 dpf, expression appears restricted in the eye (D). A lateral view (E) at 3 dpf shows expression restricted to the eye. The images in (F) and (G) show higher magnifications of the same lateral view and a ventral view, respectively. Areas lacking expression are indicated by stars (in F and G), and an arrowhead indicates expression at the choroid fissure (in F). (H) Cross-section of retina (dorsal at the top) shows expression in both dorsal and ventral CMZ (arrows), and in different layers within the dorsal retina. Stars indicate reduced expression in the ventral part of retina. (I–L) Images of the 5 dpf stage are arranged in the same way as 3 dpf (E–H). Hybridization signals persist in the dorsal-nasal retina and at the choroid fissure (arrowhead in J). Areas without staining are indicated by stars (in J and K). (L) Cross-section shows expression throughout retina including CMZ (arrows), except the ventral region marked with stars. Abbreviations: hpf, hours post-fertilization; dpf, days post-fertilization; gcl, ganglion cell layer; inl, inner nuclear layer; onl, outer nuclear layer; on, optic nerve.