FIGURE

Fig. 4

ID
ZDB-FIG-090520-16
Publication
Yeo et al., 2001 - Overexpression of a slit homologue impairs convergent extension of the mesoderm and causes cyclopia in embryonic zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Overexpression of slit2 mRNA impairs migration of the prechordal mesodermal cells and the ventral diencephalic cells in the zebrafish embryo. (A–F) The retarded migration of the prechordal plate cells in the slit2-overexpressing embryos (D–F) in comparison to normal embryos (A–C) at 100% epiboly. The positions of the mesodermal cells were examined by comparing the expression patterns of the prechordal plate mesoderm markers, zfz8b (A, D) and ,em>hlx1 (B, E), and the chordal mesoderm marker ntl (C, F) relative to the neural plate markers dlx3 (A, B, D, E), and pax2 (A, C, D, F), which are expressed in the anterior margin of the neural plate and in the midbrain/hindbrain boundary (MHB), respectively. In the slit2-overexpressing embryos, the zfz8b- and hlx1-positive prechordal mesodermal cells are prominently displaced caudally and are improperly located between the anterior margin of the neural plate and MHB. The chordal mesoderm is wider and flatter in the slit2-overexpressing embryo than in the normal embryo. (G–K) The retarded migration of the ventral diencephalic cells both in the slit2-overexpressing and cyctz219 mutant embryos at 100% epiboly. The position of the fkh5-positive diencephalic cells relative to the dlx3-positive anterior margin of the neural plate is displaced caudally in the slit2-overexpressing embryo (K) in comparison to the normal embryo (H), as in the cyctz219 mutant (I). The opl-positive cells are normally distributed in the slit2-overexpressing embryo (J) as in the normal embryo (G). (L) Schematic of the normal expression patterns of the marker genes used in this figure. np, neural plate; ms, chordal and prechordal mesoderm. Bars, 200 μm.

Expression Data
Gene:
Fish:
Anatomical Term:
Stage: Bud

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Bud

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 230(1), Yeo, S.Y., Little, M.H., Yamada, T., Miyashita, T., Halloran, M.C., Kuwada, J.Y., Huh, T.L., and Okamoto, H., Overexpression of a slit homologue impairs convergent extension of the mesoderm and causes cyclopia in embryonic zebrafish, 1-17, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.