Fig. 7
- ID
- ZDB-FIG-090515-24
- Publication
- Essner et al., 1996 - Expression of zebrafish connexin43.4 in the notochord and tail bud of wild-type and mutant no tail embryos
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Cx43.4 protein and Cx43.4 message are localized during gastrulation and early somite stages to similar regions, and the expression of a Cx43.4/GFP fusion protein during blastula stages localizes to regions of cell apposition. (A–D) Immunohistochemical localization of Cx43.4 protein. (A) Tissue from the germ ring of a shield stage embryo; side view with animal pole toward the top; arrow points to blastoderm margin. (B) Tissue from tail bud of a 1-somite stage embryo; dorsal view with posterior to the top; arrow points to blastopore. (C) Tissue from the trunk region of a 1-somite stage embryo; dorsal view with posterior to the bottom; arrow points to the notochord. (D) Scanning confocal microscopy image of the germ ring of a 70% epiboly stage embryo, side view. (E, F) Confocal microscopy images of a living zebrafish embryo after co-injection of Cx43.4/GFP mRNA and rhodamine-dextran at the 1-cell stage. (E) The Cx43.4/GFP fusion protein was visualized during the late blastula period as an extensive array of fluorescent plaques. (F) Superimposed images of the Cx43.4/ GFP protein in E and rhodamine-dextran, showing that most of the fluorescence from the fusion protein is associated with cell surfaces. Scale bar in C represents 100 μm and is the same for A and B; the scale bar in D represents 12 μm and in F, 20 μm. |
Reprinted from Developmental Biology, 177(2), Essner, J.J., Laing, J.G., Beyer, E.C., Johnson, R.G., and Hackett, P.B. Jr., Expression of zebrafish connexin43.4 in the notochord and tail bud of wild-type and mutant no tail embryos, 449-462, Copyright (1996) with permission from Elsevier. Full text @ Dev. Biol.