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ZFIN ID: ZDB-FIG-090504-37
Kalén et al., 2009 - Combination of reverse and chemical genetic screens reveals angiogenesis inhibitors and targets. Chemistry & Biology   16(4):432-441 Full text @ Chem. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. S4b Summary of Zebrafish Knock-Down Phenotypes The results from the MPO knock-down of (a) fzd6, (b) X99384, (c) ctsz, (d) rab11, (e) hexb, (f) p2ry5, (g) ralb, orthologue 1, (h) ralb, orthologue 2, (i) rab5c, (j) ppap2a, (k) ppp4c, (l) ppp1ca, (m) gnb2l1, (n) sat1, (o) ppp1cc, (p) ppih and (q) alox5ap, are listed with regards to:

The morphology at 28 and 56hpf of control and MPO knock-down zebrafish embryos.
The results from the cdh5 in situ hybridization at 48-56hpf.
The results from the FITC-dextran Microangiography at 56hpf.

The vascular defects were categorized as being Normal, Low (i.e. <30% absence of intersegmental vessel staining or FITC-Dextran signal), Medium (i.e. 31-70% defective intersegmental vessel staining/signal) or High (i.e., >70% defective intersegmental vessel staining/signal).

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-alox5ap + MO2-alox5ap standard conditions Prim-5 cell death increased occurrence, abnormal
Long-pec blood circulation disrupted, abnormal
Long-pec intersegmental vessel decreased functionality, abnormal
Long-pec pericardium edematous, abnormal
Long-pec yolk edematous, abnormal
WT + MO1-plpp1b + MO2-plpp1b standard conditions Prim-5 cell death increased occurrence, abnormal
Prim-5 yolk edematous, abnormal
Long-pec blood circulation disrupted, abnormal
Long-pec intersegmental vessel decreased functionality, abnormal
Long-pec yolk edematous, abnormal
WT + MO1-ppih + MO2-ppih standard conditions Prim-5 yolk edematous, abnormal
Long-pec blood circulation disrupted, abnormal
Long-pec intersegmental vessel decreased functionality, abnormal
Long-pec pericardium edematous, abnormal
Long-pec yolk edematous, abnormal
WT + MO1-ppp1cab + MO2-ppp1cab standard conditions Prim-5 yolk edematous, abnormal
Long-pec hindbrain distended, abnormal
Long-pec intersegmental vessel decreased functionality, abnormal
Long-pec yolk edematous, abnormal
WT + MO1-ppp1cc + MO2-ppp1cc standard conditions Prim-5 cell death increased occurrence, abnormal
Prim-5 hindbrain distended, abnormal
Prim-5 yolk edematous, abnormal
Long-pec blood circulation disrupted, abnormal
Long-pec hindbrain distended, abnormal
Long-pec intersegmental vessel decreased functionality, abnormal
Long-pec yolk edematous, abnormal
WT + MO1-ppp4ca + MO2-ppp4ca standard conditions Long-pec blood circulation disrupted, abnormal
Long-pec intersegmental vessel decreased functionality, abnormal
Long-pec yolk edematous, abnormal
WT + MO1-rack1 + MO2-rack1 standard conditions Prim-5 cell death increased occurrence, abnormal
Prim-5 fourth ventricle distended, abnormal
Prim-5 whole organism curved ventral, abnormal
Prim-5 yolk edematous, abnormal
Long-pec blood circulation disrupted, abnormal
Long-pec intersegmental vessel decreased functionality, abnormal
Long-pec yolk edematous, abnormal
WT + MO1-sat1b + MO2-sat1b standard conditions Prim-5 yolk edematous, abnormal
Long-pec blood circulation disrupted, abnormal
Long-pec intersegmental vessel decreased functionality, abnormal
Long-pec pericardium edematous, abnormal
WT + MO2-rab5c + MO3-rab5c standard conditions Prim-5 cell death increased occurrence, abnormal
Prim-5 extension decreased size, abnormal
Prim-5 hindbrain distended, abnormal
Long-pec blood vasculature broken, abnormal
Long-pec cell death increased occurrence, abnormal
Long-pec extension decreased size, abnormal
Long-pec eye decreased size, abnormal
Long-pec head decreased size, abnormal
Long-pec intersegmental vessel decreased functionality, abnormal
Long-pec whole organism curved ventral, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Chemistry & Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.
Permission Granted since 07.17.2007
Full text @ Chem. Biol.