Summary of Zebrafish Knock-Down Phenotypes The results from the MPO knock-down of (a) fzd6, (b) X99384, (c) ctsz, (d) rab11, (e) hexb, (f) p2ry5, (g) ralb, orthologue 1, (h) ralb, orthologue 2, (i) rab5c, (j) ppap2a, (k) ppp4c, (l) ppp1ca, (m) gnb2l1, (n) sat1, (o) ppp1cc, (p) ppih and (q) alox5ap, are listed with regards to:
The morphology at 28 and 56hpf of control and MPO knock-down zebrafish embryos. The vascular defects were categorized as being Normal, Low (i.e. <30% absence of intersegmental vessel staining or FITC-Dextran signal), Medium (i.e. 31-70% defective intersegmental vessel staining/signal) or High (i.e., >70% defective intersegmental vessel staining/signal).
The results from the cdh5 in situ hybridization at 48-56hpf.
The results from the FITC-dextran Microangiography at 56hpf.