ZFIN ID: ZDB-FIG-080904-54
Kai et al., 2008 - Sphingosine-1-phosphate receptors regulate individual cell behaviours underlying the directed migration of prechordal plate progenitor cells during zebrafish gastrulation. Development (Cambridge, England)   135(18):3043-3051 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 50%-epiboly

Fig. 3 mil is required for the stabilisation of cellular processes towards the direction of migration. The orientation and length of lamellipodia-like cellular processes of leading edge cells in a WT embryo (A), slb embryo (B) and slb embryo injected with 4.3 ng mil-MO (C). The morphology of a cell from each group is highlighted in the insets. The length of the long axis of cells and their orientation with respect to the direction of movement (y-axis) were calculated and plotted, and the angle from the y-axis was calculated as cosine. Statistically significant difference is indicated (##P<0.005).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
wnt11f2tx226/tx226 standard conditions 50%-epiboly mesodermal cell lamellipodium length, normal
wnt11f2tx226/tx226 + MO1-s1pr2 standard conditions 50%-epiboly mesodermal cell lamellipodium increased length, abnormal
50%-epiboly mesodermal cell migration process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development