ZFIN ID: ZDB-FIG-080904-54
Kai et al., 2008 - Sphingosine-1-phosphate receptors regulate individual cell behaviours underlying the directed migration of prechordal plate progenitor cells during zebrafish gastrulation. Development (Cambridge, England)   135(18):3043-3051 Full text @ Development
Knockdown Reagent:
Observed In:
Stage: 50%-epiboly

Fig. 3 mil is required for the stabilisation of cellular processes towards the direction of migration. The orientation and length of lamellipodia-like cellular processes of leading edge cells in a WT embryo (A), slb embryo (B) and slb embryo injected with 4.3 ng mil-MO (C). The morphology of a cell from each group is highlighted in the insets. The length of the long axis of cells and their orientation with respect to the direction of movement (y-axis) were calculated and plotted, and the angle from the y-axis was calculated as cosine. Statistically significant difference is indicated (##P<0.005).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
wnt11f2tx226/tx226 standard conditions 50%-epiboly mesodermal cell lamellipodium length, normal
wnt11f2tx226/tx226 + MO1-s1pr2 standard conditions 50%-epiboly mesodermal cell lamellipodium increased length, abnormal
50%-epiboly mesodermal cell migration process quality, abnormal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development