FIGURE

Fig. 3

ID
ZDB-FIG-080620-9
Publication
Duan et al., 1999 - Insulin-like growth factor binding protein 2 is a growth inhibitory protein conserved in zebrafish
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Fig. 3

The zebrafish IGFBP-2 cDNA encodes a protein that binds to IGFs but not to insulin and Des(1-3)IGF-I. (A) Ligand blot analysis of CM prepared from the pRc/CMV-IGFBP-2-transfected clones 2, 5, and 6 (lanes 2-4), the pRC/CMV vector-transfected clones 1 and 2 (lanes 5 and 6), and pure bovine IGFBP-2 (lane 1). (B) Affinity crosslinking of [125I] IGF-I to zebrafish IGFBP-2 produced by CHO cells (IGFBP-2-transfected clone 2). Binding was competed by the addition of no competitor (lane 2), 500 ng of IGF-I (lane 3), IGF-II (lane 4), Des(1-3)IGF-I (lane 5), and insulin (lane 6). Lane 1 is the negative control that did not have any zebrafish IGFBP-2. The competition of [125I] IGF-I was similar in all other IGFBP-2-transfected clones examined (clones 5 and 6). (C) Affinity crosslinking of [125I] IGF-II to zebrafish IGFBP-2 produced by CHO cells (IGFBP-2-transfected clone 2). Binding was competed by the addition of no competitor (lane 2), 500 ng of IGF-I (lane 3), IGF-II (lane 4), Des(1-3)IGF-I (lane 5), and insulin (lane 6). Lane 1 is the negative control that did not have any zebrafish IGFBP-2. The competition of [125I] human IGF-II was similar in other IGFBP-2-transfected clones examined (clones 5 and 6).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA