FIGURE

Fig. 3

ID

ZDB-FIG-080606-10

Publication

Pando et al., 2001 - A cell-based system that recapitulates the dynamic light-dependent regulation of the vertebrate clock

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Fig. 3

Entrainment of naive Z3 cells to a LD cycle. RNase protection analysis of Per1, Per2, Per3, Clock, Bmal1, and Bmal2 gene expression in Z3 cells maintained in constant darkness and never before exposed to a LD cycle. Naive cells were exposed to a 12:12 LD cycle, and RNA was harvested at ZT3, 9, 15, and 21. A flask of cells, synchronously prepared with the experimental samples, was continuously maintained in total darkness and harvested at the same time as the ZT3 sample of day 1, representing our dark control (d). The bar above indicates light (white) and dark (black) periods. tRNA serves as our negative control reaction (t). Relative amounts of total RNA used for each sample are displayed (RNA).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA