FIGURE

Fig. 5

ID

ZDB-FIG-080423-9

Publication

Malicki et al., 2003 - Zebrafish N-cadherin, encoded by the glass onion locus, plays an essential role in retinal patterning

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Fig. 5

Protein product localization in mutant retinae. At 24 hpf, N-cadherin polypeptide is present in wild-type (A), glo^m117 (B) but not in pac^tm101b (C) retinae. This situation persists at later stages of development as shown for the wild-type (D), glo^m117 mutants (E), and pac^tm101b mutants (F). Residual staining present in (C) and (F) is most likely due to background fluorescence. As reported previously in wild-type animals, N-cadherin is expressed in plexiform layers (arrows in D and G) and in the optic nerve (arrowheads D in G). The glo^m117 mutant polypeptide appears to retain this distribution. It is present both in the optic nerve (arrowheads in E and H) and in ectopically localized plexiform patches (arrows in E and H). Western blotting indicates that N-cadherin polypeptide is absent in pac^{tm101b/tm101b} mutant embryos (I) but it persists in glo^m117/m117 animals (J) at 3 dpf. (D, E, G, H) Retinae at 50 hpf. Retina in (F) is at 72 hpf. “le” indicates lens. Arrowheads in (A–C) indicate the midline. In (A–H) dorsal is up.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 259(1), Malicki, J., Jo, H., and Pujic, Z., Zebrafish N-cadherin, encoded by the glass onion locus, plays an essential role in retinal patterning, 95-108, Copyright (2003) with permission from Elsevier. Full text @ Dev. Biol.