ZFIN ID: ZDB-FIG-080325-7
Sperber et al., 2008 - Zebrafish dlx2a contributes to hindbrain neural crest survival, is necessary for differentiation of sensory ganglia and functions with dlx1a in maturation of the arch cartilage elements. Developmental Biology   314(1):59-70 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 2 dlx1a and dlx2a are required for proper head outgrowth and arch cartilage patterning. (A–D) Lateral views of head morphology in 5-day-old larvae that received the (A) control MO, 0.5 mM; (B) dlx1aMO, 0.5 mM; (C) dlx2aMO, 0.25 mM; (D) an equal combination of dlx1aMO + dlx2aMO 0.25 mM each. (E–P) Cartilage elements stained with alcian blue. (E–H) are lateral views; (I–L) are ventral view. The inset in panel H is a lateral oblique view showing loss of the interhyal by fusion of hyosymplectic and ceratohyal elements (arrowhead). (M–P) Dissected ceratobranchial arch elements, numbered. cb, ceratobranchials; ch, ceratohyal; eth, ethmoid plate; hys, hyosymplectic; ih, interhyal; M, Meckel′s cartilage; pq, palatoquadrate. Arrows (A–D) indicate position of the mouth; asterisk in panel P indicates site of lateral branching. (A–P) Anterior is to the left. Scale bar in panel A: 250 µm; panels E and I: 100 μm; panel H inset, and M: 50 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dlx1a standard conditions Day 5 ceratobranchial cartilage chondrocyte decreased amount, abnormal
Day 5 ceratohyal cartilage deformed, abnormal
Day 5 ethmoid cartilage decreased size, abnormal
Day 5 ethmoid cartilage shape, normal
Day 5 head decreased size, abnormal
Day 5 hyosymplectic cartilage deformed, abnormal
Day 5 Meckel's cartilage deformed, abnormal
Day 5 palatoquadrate cartilage deformed, abnormal
Day 5 pharyngeal arch cartilage decreased size, abnormal
Day 5 pharyngeal arch cartilage deformed, abnormal
Day 5 trabecula cranii decreased size, abnormal
Day 5 trabecula cranii shape, normal
WT + MO1-dlx1a + MO2-dlx2a standard conditions Day 5 ceratobranchial cartilage chondrocyte decreased amount, abnormal
Day 5 ceratobranchial cartilage deformed, abnormal
Day 5 ceratohyal cartilage deformed, abnormal
Day 5 ethmoid cartilage decreased size, abnormal
Day 5 ethmoid cartilage shape, normal
Day 5 head decreased size, abnormal
Day 5 hyosymplectic cartilage deformed, abnormal
Day 5 interhyal cartilage aplastic, abnormal
Day 5 pharyngeal arch cartilage chondrocyte decreased amount, abnormal
Day 5 pharyngeal arch cartilage decreased size, abnormal
Day 5 pharyngeal arch cartilage deformed, abnormal
Day 5 trabecula cranii decreased size, abnormal
Day 5 trabecula cranii shape, normal
WT + MO2-dlx2a standard conditions Day 5 ceratobranchial cartilage chondrocyte decreased amount, abnormal
Day 5 ceratohyal cartilage deformed, abnormal
Day 5 ethmoid cartilage decreased size, abnormal
Day 5 ethmoid cartilage shape, normal
Day 5 head decreased size, abnormal
Day 5 hyosymplectic cartilage deformed, abnormal
Day 5 Meckel's cartilage deformed, abnormal
Day 5 palatoquadrate cartilage deformed, abnormal
Day 5 pharyngeal arch cartilage decreased size, abnormal
Day 5 pharyngeal arch cartilage deformed, abnormal
Day 5 trabecula cranii decreased size, abnormal
Day 5 trabecula cranii shape, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 314(1), Sperber, S.M., Saxena, V., Hatch, G., and Ekker, M., Zebrafish dlx2a contributes to hindbrain neural crest survival, is necessary for differentiation of sensory ganglia and functions with dlx1a in maturation of the arch cartilage elements, 59-70, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.