ZFIN ID: ZDB-FIG-070813-32
Hutchinson et al., 2007 - Nkx6 proteins specify one zebrafish primary motoneuron subtype by regulating late islet1 expression. Development (Cambridge, England)   134(9):1671-1677 Full text @ Development
Anatomical Term:
Stage Range: Prim-5 to Prim-25

Fig. S1 Zebrafish have three Nkx6 proteins. (A) Nkx phylogeny demonstrating the three zebrafish Nkx6 proteins uniquely cluster within one node which is distinct from the Nkx2.2 and Nkx1 class of proteins. The PHYLIP software package was employed to create a neighbor-joining tree using the ClustalW algorithm. Sequences included in the tree were gathered from GenBank and bootstrap values are indicated. Expression of nkx6.3 mRNA begins at 20 hpf, persists until at least 72 hpf, and is limited to a small population of cells posterior to rhombomere 5 in the hindbrain. (B) Alignment of zebrafish Nkx6.1, Nkx6.2 and Nkx6.3 translated amino acid sequences. The dashed red line indicates the NK domain and the solid blue line indicates the homeodomain. (C) Whole-mount in situ RNA hybridization reveals that nkx6.3 is expressed in a subset of hindbrain cells at 25 hpf. (D) At 36 hpf, nkx6.3-expressing cells (purple) are Islet:GFP-negative and are located ventral to cells labeled with Islet:GFP (brown, GFP antibody staining). Lateral views, anterior to the left, dorsal to the top. ov, otic vesicle; sb, somite boundary. Scale bar: 40 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nkx6.3 AB standard conditions Prim-5 hindbrain ISH
Prim-25 hindbrain ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development