Fig. 6

Morpholino-mediated reduction of Pard3 expression caused patchy RPE pigmentation and cyclopia. A wild-type and anti-pard3 morpholino-injected embryo at 2 dpf (A and B, respectively). The patchy pigmentation in the RPE (arrow) and the pericardial edema (arrowhead) in the morpholino-treated embryo are marked. The ventral head region of a wild-type embryo (C) shows the normal spacing between the eyes. A spectrum of cyclopic phenotypes (D–H) in anti-pard3 morpholino-treated embryos exist from the wild-type distance between the eyes (D) to a single eye (H). The numbers in the lower right corner of Panels D–H (ranging from 1 to 5, respectively) represent the degrees of cyclopia expressivity that were used to determine the cyclopia index in Table 2 and Table 3. Co-injection of in vitro-transcribed pard3 mRNA (encoding the 150-kDa Pard3 isoform flanked by the β-globin 5′ and 3′ UTRs) with the anti-pard3 morpholino (Morph-1) rescued the Morph-1-induced cyclopia phenotype and the patchy RPE pigmentation defect (I). Scale bars indicate 100 μm.