FIGURE

Fig. 4

ID

ZDB-FIG-060410-24

Publication

von Trotha et al., 2006 - Apical localization of ASIP/PAR-3:EGFP in zebrafish neuroepithelial cells involves the oligomerization domain CR1, the PDZ domains, and the C-terminal portion of the protein

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Fig. 4

The apical localization signals in ASIP/PAR-3 prevent the basolateral localization of a Numb:PAR-3 fusion protein. A-C: Localization of the different EGFP-tagged Numb:ASIP fusion constructs. See text for details. A,B: Apical membrane localization is denoted by arrows, whereas arrowheads point to a localization at the basolateral cell cortex. Note: Decreased resolution in B is due to a shorter confocal scanning time. C: Numb(1-196):ASIP(658-1127):EGFP is mainly localized to the basolateral membrane (arrowhead), resulting in an apical cortex that is almost completely devoid of the fusion protein (arrow). Anterior is toward the upper left. Scale bar = 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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