Fig. 1

Phenotype of oep^m134 and oep^z1 mutant embryos. (A-D) Comparison of wild-type (A), homozygous oep^m134 (B), homozygous oep^z1 (C) and transheterozygous oep^m134/oep^z1 (D) embryos at 27-28 hpf in a lateral view. Here and in all other figures anterior is to the left and dorsal is up, except where indicated otherwise. Note the general degeneration of the head region of homozygous oep^z1 mutant embryos (arrow in C) but not homozygous oep^m134 or transheterozygous oep^m134/oep^z1. (E-H) Comparison of the head region of wild-type (E), homozygous oep^m134 (F), homozygous oep^z1 (G) and transheterozygous oep^m134/oep^z1 (H) embryos at 27-30 hpf in a lateral view. Note the anterior-medial location of the single eye in oep mutants (arrow). + indicates the location of the midbrain-hindbrain boundary. (I,J) Ventral view of the head region of wild-type (I) and oep^m134 mutant (J) embryos at 27-29 hpf. Arrows highlight the location of the two eyes in wild-type and the single median eye in oep mutant embryos. (K,L) Hatching gland formation over the yolk in wild-type (arrow in K) but not oep^m134 mutants (L) at 38 hpf. Ventral view, anterior is up. (M-T) Notochord formation in wild-type (M,Q), oep^m134 (N,P,R,T) and oep^z1 (O,S) mutant embryos at 27-30 hpf. (M-P) Dorsal view of the anterior-most region of the notochord (outlined by dots). Note that the anterior extent of the notochord is normally found next to the otic vesicle (indicated by stars). In some oep mutants the anterior end of the notochord is located more posteriorly (P). (Q-T) Lateral view of the notochord in the trunk and tail region. Arrows outline the borders of the notochord. The floor plate and neurocoel lie directly dorsal to the notochord. Note the wavy and thinner appearance of the notochord in some oep mutants (T).