EST

cb489

ID
ZDB-EST-030429-36
Name
cb489
Symbol
cb489
Previous Names
None
Type
EST
Location
Unmapped
Genome Resources
None
Species
Danio rerio
Library
oligo-dT primed 26-somite stage
Digest
PCR amplification
Insert Size
4500
Cloning Site
EcoRI-SalI
Vector Type
Plasmid
Vector
pSPORT1
Polymerase
T3 RNA polymerase
PCR Amplification
(replaces the SP6 promoter with a T3 promoter)
T3p : 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3'
T7p : 5' TAA TAC GAC TCA CTA TAG GG 3'
Suppliers
None
Quality
( Nice strong expression pattern )
Protocol
Thisse in situ hybridization protocol
Note
None
Gene Expression
Directly Submitted Expression Data
6 figures (34 images) from Thisse et al., 2001 [cb489]
Marker Relationships
Sequences
Citations