EST Name: cb609
Sequence Ontology ID : SO:0000345
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Species: Danio rerio
Library: oligo-dT primed shield stage
Digest: PCR amplification
Insert Size: 1200
Cloning Site: EcoRI-SalI
Vector Type: Plasmid
Vector: pSPORT1
Polymerase: T3 RNA polymerase
PCR Amplification: (replaces the SP6 promoter with a T3 promoter)
T3p : 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3'
T7p : 5' TAA TAC GAC TCA CTA TAG GG 3'
Source:
Quality: Rating 2 ( Weak expression pattern )
Protocol: Thisse in situ hybridization protocol
Location: Unmapped
Note: CB609 is an EST corresponding to a gene which encodes a Glyceraldehyde-3-phosphate dehydrogenase expressed in YSL and muscles and which is different from the previously reported Glyceraldehyde-3-phosphate dehydrogenase gene corresponding to the EST CB350 and which is expressed in neurons.
GBROWSE
GENE EXPRESSION
Directly Submitted Expression Data: 6 figures (28 images) from Thisse et al., 2001 [cb609]
MARKER RELATIONSHIPS
cb609 Is Encoded by: [Gene] gapdh (1)
SEQUENCE INFORMATION
Type Accession # Length (nt/aa) Analysis
RNA GenBank:CA854263 (1) 650 nt
Genome Resources:
CITATIONS (2)