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General Information
ZIRC
ZFIN ID: ZDB-EST-030116-10
EST Name: cb619
Sequence Ontology ID : SO:0000345
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(Including Attributions)
Species: Danio rerio
Library: oligo-dT primed shield stage
Digest: PCR amplification
Insert Size: 1300
Cloning Site: EcoRI-SalI
Vector Type: Plasmid
Vector: pSPORT1
Polymerase: T3 RNA polymerase
PCR Amplification: (replaces the SP6 promoter with a T3 promoter)
T3p : 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3'
T7p : 5' TAA TAC GAC TCA CTA TAG GG 3'
Source:
Quality: Rating 5 ( Simple to use, intense expression pattern restricted to a few structures )
Protocol: Thisse in situ hybridization protocol
Location: Unmapped
GBROWSE
GENE EXPRESSION
Directly Submitted Expression Data: 6 figures (31 images) from Thisse et al., 2001 [cb619]
MARKER RELATIONSHIPS
cb619 Is Encoded by: [Gene] gbx1 (1)
SEQUENCE INFORMATION
Type Accession # Length (bp/aa) Analysis
RNA GenBank:CA588089 (1) 681 bp
Sequence Information (all 2)
Genome Resources:
CITATIONS (2)