| EST Name: |
cb619
|
| Sequence Ontology ID : |
SO:0000345 |
|
Previous Name:
|
|
Attributions for Alias: {{control.newAlias}}
Delete Alias:
(Including Attributions)
|
| Species: |
Danio rerio |
| Library: |
oligo-dT primed shield stage |
| Digest: |
PCR amplification |
| Insert Size: |
1300 |
| Cloning Site: |
EcoRI-SalI |
| Vector Type: |
Plasmid |
| Vector: |
pSPORT1 |
| Polymerase: |
T3 RNA polymerase |
| PCR Amplification: |
(replaces the SP6 promoter with a T3 promoter)
T3p : 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3'
T7p : 5' TAA TAC GAC TCA CTA TAG GG 3' |
| Source: |
|
|
Quality:
|
(
Simple to use, intense expression pattern restricted to a few structures
)
|
| Protocol: |
Thisse in situ hybridization protocol
|
| Location: |
Unmapped
|
