EST
cb427
- ID
- ZDB-EST-021210-5
- Name
- cb427
- Symbol
- cb427
- Previous Names
- None
- Type
- EST
- Location
- Unmapped
- Genome Resources
- None
- Species
- Danio rerio
- Library
- oligo-dT primed 26-somite stage
- Digest
- PCR amplification
- Insert Size
- 1300
- Cloning Site
- EcoRI-SalI
- Vector Type
- Plasmid
- Vector
- pSPORT1
- Polymerase
- T3 RNA polymerase
- PCR Amplification
-
(replaces the SP6 promoter with a T3 promoter)
T3p : 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3'
T7p : 5' TAA TAC GAC TCA CTA TAG GG 3' - Suppliers
- Quality
- ( Simple to use, intense expression pattern restricted to a few structures )
- Protocol
- Thisse in situ hybridization protocol
- Note
-
EGR2 is a marker of rhombomere 3 and 5 of hindbrain from late gastrulation until hatching
Gene Expression
- Directly Submitted Expression Data
- 6 figures (15 images) from Thisse et al., 2001 [cb427]
Marker Relationships
Sequences
Citations