ZFIN ID: ZDB-EST-021024-18
EST Name: cb436
Sequence Ontology ID : SO:0000345
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Species: Danio rerio
Library: oligo-dT primed 26-somite stage
Digest: PCR amplification
Insert Size: 3800
Cloning Site: EcoRI-SalI
Vector Type: Plasmid
Vector: pSPORT1
Polymerase: T3 RNA polymerase
PCR Amplification: (replaces the SP6 promoter with a T3 promoter)
T3p : 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3'
T7p : 5' TAA TAC GAC TCA CTA TAG GG 3'
Source:
Zebrafish International Resource Center (ZIRC) (order this)
Quality: Rating 5 ( Simple to use, intense expression pattern restricted to a few structures )
Protocol: Thisse in situ hybridization protocol
Location: Unmapped
GBROWSE
GENE EXPRESSION
Directly Submitted Expression Data: 6 figures (23 images) from Thisse et al., 2001 [cb436]
MARKER RELATIONSHIPS
cb436 Is Encoded by: [Gene] rgs4 (1)
SEQUENCE INFORMATION
Type Accession # Length (bp/aa) Analysis
RNA GenBank:BU670721 671bp
OTHER cb436 EST PAGESNo links to external sites
CITATIONS (1)