ZFIN ID: ZDB-EST-020912-35
EST Name: cb397
Sequence Ontology ID : SO:0000345
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Species: Danio rerio
Library: oligo-dT primed 26-somite stage
Digest: PCR amplification
Insert Size: 2400
Cloning Site: NotI-SalI
Vector Type: Plasmid
Vector: pSPORT
Polymerase: T3 RNA polymerase
PCR Amplification: 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3'
5' TAA TAC GAC TCA CTA TAG GG 3'
Source:
Zebrafish International Resource Center (ZIRC) (order this)
Quality: Rating 5 ( Simple to use, intense expression pattern restricted to a few structures )
Protocol: Thisse in situ hybridization protocol
Location: Unmapped
Note: Due to the poor incorporation of DIG-NTP by the SP6 RNA polymerase, antisense RNA probes are synthesized from a PCR amplified fragment in which a T3 RNA polymerase replace the SP6 RNA polymerase promoter.
GBROWSE
GENE EXPRESSION
Directly Submitted Expression Data: 5 figures (15 images) from Thisse et al., 2001 [cb397]
MARKER RELATIONSHIPS
cb397 Is Encoded by: [Gene] pmela (1)
SEQUENCE INFORMATION
Type Accession # Length (bp/aa) Analysis
RNA GenBank:BU492980 659bp
OTHER cb397 EST PAGESNo links to external sites
CITATIONS (1)