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General Information
ZIRC
ZFIN ID: ZDB-EST-020912-20
EST Name: cb377
Sequence Ontology ID : SO:0000345
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(Including Attributions)
Species: Danio rerio
Library: oligo-dT primed 26-somite stage
Digest: PCR amplification
Insert Size: 1300
Cloning Site: NotI-SalI
Vector Type: Plasmid
Vector: pSPORT
Polymerase: T3 RNA polymerase
PCR Amplification: 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3'
5' TAA TAC GAC TCA CTA TAG GG 3'
Source:
Quality: Rating 4 ( Nice strong expression pattern )
Protocol: Thisse in situ hybridization protocol
Location: Unmapped
Note: Due to the poor incorporation of DIG-NTP by the SP6 RNA polymerase, antisense RNA probes are synthesized from a PCR amplified fragment in which a T3 RNA polymerase replace the SP6 RNA polymerase promoter.
GBROWSE
GENE EXPRESSION
Directly Submitted Expression Data: 6 figures (12 images) from Thisse et al., 2001 [cb377]
MARKER RELATIONSHIPS
cb377 Is Encoded by: [Gene] colec12 (1)
SEQUENCE INFORMATION
Type Accession # Length (bp/aa) Analysis
RNA GenBank:BU492961 695bp
Sequence Information (all 2)
OTHER cb377 EST PAGESNo links to external sites
CITATIONS (1)